Journal: Radiation Oncology (London, England)

Article Title: Resveratrol enhances prostate cancer cell response to ionizing radiation. Modulation of the AMPK, Akt and mTOR pathways

doi: 10.1186/1748-717X-6-144

Figure Lengend Snippet: Effects of resveratrol (RSV) and ionizing radiation (IR) on survival of prostate cancer (PrCa) cells . A . Cells were incubated with increasing doses of RSV (0-10 μM) and allowed to form colonies. Colonies (> 50 cells) were counted. Results are expressed as the surviving fraction compared to untreated control (see Methods) . '*' p < 0.05 compared to untreated PC3 cells, '#' p < 0.05 compared to untreated 22RV1 cells, '+' p < 0.05 compared to untreated PNT1A cells. B . Cells were pre-treated with RSV (0-10 μM) followed by treatment with a single fraction of IR (2 Gy) and allowed to grow and form colonies. Treatment values were normalized to untreated controls and surviving fractions are presented as percent of control. Shown is the mean of 4-5 independent experiments ± standard error (SE). '*' p < 0.05 compared to 2 Gy treated PC3 cells, '#' p < 0.05 compared to 2 Gy treated 22RV1 cells, '+' p < 0.05 compared to 2 Gy treated PNT1A cells. C . Projected proportion of tumour survival after treatment with 0 - 80 Gy was estimated using the Surviving Fraction at 2 Gy (SF2) values for control and RSV (5 μM) treated PNT1A, PC3 and 22RV1 cells. SF2 n was determined as the tumour proportion surviving after "n" 2 Gy fractions, assuming iso-effectiveness of each fraction. Values were plotted up to a scale of 1 × 10 -11 to reflect elimination of tumour of 50 - 100 gr expected to contain 5 - 10 × 10 10 cells.

Article Snippet: Human PrCa (PC3, 22RV1) and normal prostate epithelial (PNT1A) cell lines were obtained from American Tissue Culture Collection (Manassas, VA, U.S.A.).

Techniques: Incubation, Control

Journal: Molecular and cellular endocrinology

Article Title: Stanniocalcin-1 protein expression profile and mechanisms in proliferation and cell death pathways in prostate cancer.

doi: 10.1016/j.mce.2019.110659

Figure Lengend Snippet: Figure 2. STC1 gene and protein expression in PC-3, LNCaP and PNT1A cell lines. (A) Gene expression of STC1 in human prostate cell lines. STC1 was not detectable in PNT1A. The bars represent the mean of 3 independent experiments performed in triplicate ± standard deviation of the mean, "**" p < 0.01. Immunocytochemistry of STC1 protein in prostatic cells (B) PC-3, (D) LNCaP and (F) non-neoplastic prostate cells PNT1A. STC1 is stained in green and the nuclei are stained in blue. Negative control samples (only incubated with goat anti-rabbit secondary antibody) of each tissue were shown in (C), (E), and (G).

Article Snippet: Cell lines and culture maintenance The prostate cancer cell lines PC-3 (ATCC® CRL-1435TM) and LNCaP (ATCC® CRL-1740TM) and the normal human prostate cell line PNT1A (ECACC 9501e 2614) (#Hs 505.T, ATCC® CRL-7306TM) were maintained in RPMI medium (Gibco, BRL) supplemented with 10% fetal bovine serum (FBS, Gibco, BRL) and 0.5 mg/mL kanamycin (Invitrogen) at 37 °C in a humidified atmosphere 5 % CO2 incubator.

Techniques: Expressing, Gene Expression, Standard Deviation, Immunocytochemistry, Staining, Negative Control, Incubation

Journal: The Journal of Biological Chemistry

Article Title: Leucine-rich repeat–containing G protein–coupled receptor 4 (Lgr4) is necessary for prostate cancer metastasis via epithelial–mesenchymal transition

doi: 10.1074/jbc.M116.771931

Figure Lengend Snippet: LGR4 is overexpressed in human prostate cancer and positively correlated with cancer recurrence. A and B, LGR4 expression was assessed by qPCR (A) and Western blotting (n = 3) (B) in different human prostate cancer cell lines. C, the LGR4 mRNA level was analyzed using GEO accession no. GSE6099 (33). p = 0.007. D, the Kaplan–Meier disease-free survival curve was generated using GEO accession no. GSE3933 (34). LGR4low, n = 28; LGR4high, n = 30. E, the Kaplan–Meier disease-free survival curve was generated by using GEO accession no. GSE10645 (35). p < 10E−6; LGR4low, n = 59; LGR4high, n = 60. The data in A are expressed as means ± S.E. of three independent experiments (*, p < 0.05; **, p < 0.01; versus PNT1A control).

Article Snippet: Cell culture Human prostate cancer LNCAP, PC3, DU145, MDA-PCa-2a, MDA-PCa-2b, LAPC-4, and VCAP cells and PNT1A human normal prostate epithelial cells were purchased from the ATCC and cultured as described previously ( 56 ) without contamination such as mycoplasma.

Techniques: Expressing, Western Blot, Generated, Control

Journal: PLoS ONE

Article Title: Synthesis and characterization of novel ssDNA X-aptamers targeting Growth Hormone Releasing Hormone (GHRH)

doi: 10.1371/journal.pone.0260144

Figure Lengend Snippet: A GHRH expression was figured out in MIA PaCa-2 pancreatic cancer, HT-29 colorectal cancer, PC3 prostate cancer, and PNT1a normal prostate epithelium cell lines by immunofluorescence. DAPI was used to observe the nuclei of the cells. B Translational expression of GHRH in MIA PaCa-2, HT-29, PC3, and PNT1a cells was determined by immunoblotting. ß-actin was used as a loading control. C. GHRH concentrations in media of MIA PaCa-2, HT-29, PC3, and PNT1a cells determined by Human GHRH ELISA.

Article Snippet: HEK293 (CRL-1573), MIA PaCa-2 (CRL-1429), HT-29 (HTB-38), PC3 (CRL-1435), LNCaP (CRL-1740), and PNT1a (CRL-11609) cells were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing, Immunofluorescence, Western Blot, Control, Enzyme-linked Immunosorbent Assay